Biotechflow EBA MAb Purification from Unclarified Harvest

Tests to evaluate the performance of the EBA 300 Column using Rhobust® MAbDirect ProteinA were conducted at a cGMP manufacturing facility: High cell-density harvest material was used under the following conditions:- Cell density: 60-90 million cells/mL ~viability greater than 80% Antibody titre: 1.3 g/L, continuous XD®, cumulative titre 5.8 g/L over 21 days Load ratio: ca.22 mg IgG/mL settled bed, both Protein A resins The results showed that in comparison to the traditional clarification and chromatography processes, this EBA technology r...

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